Evidence of apoptosis and pyroptosis as mechanisms of cell death in duodenal mucosa in active Celiac Disease

RUERA CAROLINA; FEDERICO PEREZ; LUCIANA GUZMAN; LORENA MENENDEZ; LAURA GARBI; FERNANDO CHIRDO

Mar del Plata

Congreso; Reunión conjunta SAIC SAI SAFIS 2018; 2018

SAIC SAI SAFIS

Celiac disease (CD) is a chronic enteropathy characterized by massive loss of enterocytes from the proximal small intestine. Apoptosis has been considered as the main pathway of cell death, however this mechanism has been only partially studied.The objective of this work was to characterize the cell death pathways in intestinal mucosaDuodenal biopsies were collected from pediatric and adult patients during the routine procedure for CD diagnosis. Ethic committees approved this work in Public Health Institutions. Levels of caspase 1, 3, 8, 4 and Gasdermine D (GASDMD) were assessed by Western blot (WB) in protein extracts from whole biopsy. TUNEL reaction and Immunofluorescence microscopy (IFI) analysis were performed on sections of paraffin-embedded tissues.By TUNEL reaction, which shows an advanced stage in cell death, increased number of TUNEL+ cells in the lamina propria of CD patients (n=6) compared with healthy controls (n = 6) were found (p