ANNEXIN A1 EXPRESSION IN THE SMALL INTESTINE. ANALYSIS IN A MURINE MODEL OF ENTEROPATHY AND CELIAC DISEASE PATIENTS.

CAROLINA N. RUERA; MICULÁN, EMANUEL GONZALO; IRIBARREN, MARÍA LUZ; GERONIMO DUCCA; GUZMAN, LUCIANA; MENENDEZ, LORENA; GARBI LAURA; CHIRDO FERNANDO

San Luis

Congreso; LXXI Reunión anual de la sociedad argentina de inmunología; 2023

Sociedad argentina de inmunología (SAI)

ANNEXIN A1 EXPRESSION IN THE SMALL INTESTINE. ANALYSIS INA MURINE MODEL OF ENTEROPATHY AND CELIAC DISEASE PATIENTSCarolina Ruera1, Emanuel Miculan1, María Luz Iribarren1, Gerónimo Ducca1, LucianaGuzman2, Lorena Menendez2, Laura Garbi3 and Fernando Chirdo1Instituto de Estudios Inmunológicos y Fisiopatológicos - IIFP (UNLP-CONICET) 1, Servicio deGastroenterología del Hospital de Niños2 y San Martin3. National University of La Plata. La Plata.Argentina.Objective: Annexin A1 (ANXA1) participates in the control of immune response,apoptosis, cell differentiation and modulation of the inflammatory response, andhas been implicated in a variety of chronic inflammatory diseases. Although, theanti-inflammatory role of ANXA1 was demonstrated in colitis model of dextransodium sulphate, and its expression has been described in the small intestine,there are no studies on its functional role in this tissue. ANXA1 is found indifferent cell types, including T lymphocytes and neutrophils expressingmyeloperoxidase (MPO).In previous work, we showed the activation of inflammatory markers andapoptosis in small intestine mucosa of Celiac Disease (CD) patients. CD is ahighly prevalent chronic enteropathy driven by dietary gluten in geneticallysusceptible individuals.This study aimed to assess the expression of ANXA1 in the small intestine in amurine model of enteropathy and small intestinal samples of CD patients.Methods: 8 weeks old C57BL/6 wild type mice were treated by intragastricadministration of 20µg/mouse of p31-43 gliadin peptide, following a protocolalready established by our group and approved by CICUAL. Small intestineswere collected at 16h after treatment. Human duodenal biopsies were collectedduring the procedure for CD diagnosis, following Ethic approved protocols.Small intestine samples were used for confocal immunofluorescencemicroscopy (IFI) studies and western blot (WB) analysis.Results: Increased numbers of dead cells were detected by TUNEL reaction inboth duodenum of untreated CD patients and in proximal small intestine of miceorally challenged with p31-43 (p