Coexistence of apoptosis, pyroptosis, and necroptosis pathways in Celiac Disease

RUERA, CAROLINA NAYMÉ; FEDERICO PEREZ; IRIBARREN MARÍA LUZ; LUCIANA GUZMAN; LORENA MENENDEZ; LAURA GARBI; CHIRDO FERNANDO

Wegeningen, The Netherlands

Encuentro; 36rd Meeting of the European Prolamin Working Group of Prolamin Analysis and Toxicity; 2023

European Prolamin Working Group of Prolamin Analysis and Toxicity

Regulated cell death incudes different pathways and diverse outcomes for the tissue.Particularly, apoptosis, considered as an immunologically silent process, is the cell deathpathway commonly used for the massive elimination of cells, such as enterocytes in CoeliacDisease (CD). Unlike apoptosis, pyroptosis and necroptosis results in the release of intracellularcomponents with proinflammatory activity, which may expand the inflammation and tissuedamage. However, little is known about the role of proinflammatory cell death pathways inhuman intestinal disease.The aim of this work was to evaluate the expression of components of proinflammatory celldeath pathways in healthy human small intestine and duodenal samples from CD patients.Duodenal biopsies were collected from active CD patients and non-coeliac individuals duringthe routine protocol for CD diagnosis in the Gastroenterology Units of the paediatric and adultpublic hospitals from La Plata city. CD was diagnosed by histological examination of duodenalbiopsies and serological assessment. The individuals in the control group (NC) were subjectedto endoscopy for reasons other than CD and all were negative for serological or histologicalevidence of CD. Samples were used for confocal microscopy studies such as TUNEL reactionor Immunofluorescent microscopy (IFI), Western blot (WB), and RT-PCR analysis.TUNEL reaction showed an increased number of dead cells in the mucosal lamina propria (LP)of CD patients (p