POTENTIAL ROLE OF NECROPTOSIS IN THE PATHOGENESIS OF CELIAC DISEASE

CAROLINA N. RUERA; FEDERICO PEREZ; IRIBARREN MARÍA LUZ; GUZMAN LUCIANA; LORENA MENENDEZ; GARBI LAURA; FERNANDO CHIRDO

Mar del plata

Congreso; LXX Reunión anual de la sociedad argentina de inmunología; 2022

Sociedad argentina de inmunología

Apoptosis is the cell death pathway commonly used for the massive elimination of cells, such as enterocytes in celiac disease (CD), an enteropathy developed in genetically predisposed individuals after gluten ingestion. Unlike the immunologically silent apoptosis, necroptosis results in the release of intracellular components with proinflammatory activity (danger signals), which may expand the inflammation and tissue damage. The aim of this work was to evaluate the expression of components of the necroptosis pathway in healthy human small intestine and samples from CD patients.Duodenal biopsies were collected from pediatric and adult patients during the routine procedure for CD diagnosis and used for confocal microscopy studies, Western blot (WB), and RTPCR analysis. Ethics committees from Health Institutions approved this protocol. Results. TUNEL reaction showed an increased number of dead cells in the duodenum of CD patients compared withhealthy controls (p< 0,002). TUNEL+ cells were CD95+ and in close contact with CD3+ cells. In addition, p-MLKL expression was found in some CD3+ T cells in lamina propria of CD patients and Paneth cells (βdefensin+) in the crypts. WB and inmunofluorescence analysis showed an increment in RIPK3 and p-MLKL expression in CD patients (p