MILK LIPID COMPOSITION IS MODIFIED BY PERINATAL EXPOSURE TO BISPHENOL A

GABRIELA A. ALTAMIRANO; AYELEN GOMEZ; MELISA DELCONTE; ENRIQUE H. LUQUE; MÓNICA MUÑOZ-DE-TORO; LAURA KASS

Chascomús

Congreso; XVI JORNADAS ANUALES DE LA SOCIEDAD ARGENTINA DE BIOLOGÍA; 2014

Sociedad Argentina de Biología

Previously, we demonstrated that perinatal exposure to Bisphenol A (BPA) modifies milk protein composition in lactating rats. Here, the effects of perinatal exposure to BPA on milk lipid composition were analyzed during lactation. Pregnant rats were orally exposed to 0, 0.6 or 52 µg BPA/kg/day from gestation day 9 until weaning. After puberty, F1 females were bred and, on lactation day 2 (LD2) and LD10 mammary glands were obtained. On LD10, milk samples were collected, fatty acid (FA) profile and lipid composition were established. Cryostat sections were stained with Oil Red O to quantify the area of secreted milk fat globules (MFGs) within the alveolar lumen. On LD2, MFGs in samples from both BPA groups were smaller than in control animals, whereas no changes in the growth curves were observed in BPA F2 pups. On LD10, MFGs area remained smaller in BPA0.6 F1 dams than in controls but it was significantly increased in BPA52 F1 dams. Milk lipid content and FA profile of BPA-exposed animals were associated with MFGs changes. BPA0.6 F1 dams had lower whereas BPA52 F1 dams had higher concentration of lipids than control rats. Besides, body weight gain in BPA52 F2 pups was higher than in the other pups. Our results demonstrate that milk quality, evaluated by protein and lipid content is affected by the perinatal exposure to BPA, compromising the normal growth of the offspring.