DAM EARLY FREE ACCESS TO HYPERTONIC NACL SOLUTION INDUCES A LONG-TERM EFFECT ON RENAL GENE EXPRESSION IN THE ADULT OFFSPRING.

MACAGNO, AGUSTINA; PORCARI, CINTIA; VIVAS, LAURA; GODINO, ANDREA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

SAIC SAFIS SAI

Exposure to hyperosmotic environment during a pre/postnatal period can differentially program the fluid intake and excretion pattern in a way that persists until adulthood. Our results indicated that maternal voluntary ingestion of hypertonic NaCl solution during pregnancy and lactation until one-week post-weaning affect the sodium and water intake and the brain angiotensinergic type 1a receptor (AT1a) and vasopressin (AVP) mRNA expression induced by osmotic challenges. In order to analyze possible renal alterations, we studied the effect of this perinatal hypertonic sodium exposure on adult offspring kidney mRNA expression of AT1a, AVP type V2 (AVP2) and the TRPV1 channel, during basal condition and after an osmotic challenge.We studied Wistar Rats, with a perinatal manipulation (M) that covered dams from 1 week before conception until the 28 postnatal day of the offspring. The experimental groups were: -M-NaCl: Freeaccess to 0.45M NaCl solution, food and water; and -M-Ctrol: Free access to food and water. We analyzed the kidney weigh and mRNA expression of adult offspring in basal and after 2M NaCl infusion challenge conditions.We did not find any significant difference in the kidney weights between the groups. However, the M-NaCl group present a significant increase in the At1a expression in relation to M-Ctrol (F= 8.09;p=0.017, program effect). By the other hand, we found that 2M NaCl infused animals, present a significant decrease (F=6.31; p=0.026) of the TRPV1 mRNA expression (postnatal treatment effect). Finally, the AVP2 receptor gene expression did not show a significant difference but had a tendency to increase in M-NaCl animals (p=0.08).Together these and our previous results indicate that the availability of a rich source of NaCl during the perinatal period induces longterm changes at brain and renal angiotensinergic and vasopressinergic systems modulating the behavioral, endocrine and renal response to achieve the hydroelectrolyte homeostasis.