Dam Early Free Access to Hypertonic NaCl Solution Induces a Long-Term Effect on Offspring Basal Chronic Brain Cell Activity

MACAGNO, AGUSTINA; GODINO, ANDREA; PORCARI, CINTIA; MACCHIONE AF; CAEIRO X; VIVAS L.

Havana

Congreso; 2nd Pan-American Congress of Physiological Sciences, PANAM 2019; 2019

Exposure to an altered osmotic environment during a pre/postnatal period candifferentially program the fluid intake and excretion pattern profile in a way that persistsuntil adulthood. Our recent results indicate that maternal voluntary ingestion ofhypertonic NaCl solution during pregnancy and lactation until one week post-weaningalters the offspring?s central osmoregulatory mechanisms. Offspring water and sodiumintake induced by different osmotic manipulations (sodium depletion or sodiumoverload) is changed during adulthood (Macchione et al., 2012; 2015). However, theanalysis of the programing effects on basal pattern of neuronal activity along brainnuclei involved in the control of hydroelectrolyte balance is limited. Our aim was toevaluate the impact of maternal voluntary ingestion of hypertonic NaCl solution (aspreviously detailed) in the basal brain chronic neuronal activity of adult?s offspring.Animal handling and experimental procedures were approved by the Animal Care andUse Committee of our institute, and the National Institutes of Health (NIH) Guidelineswere followed. We analyzed the immunohistochemical detection of brain Fra likeprotein (Fra-LI), alone or combined with vasopressin (AVP) in control (M-Ctrol group)and imprinted (M-Na group) rats, at 70 postnatal day.Our results indicate that, the M-Na group showed increased Fra-Li immunoreactivity (ir)along the organum vasculosum of the lamina terminalis (OLVT) (M-Ctrol= 105.75±21.77 vs M-Na= 308.5 ±59.01; p=0.018; t=-3.22). However, double Fra-AVP ir neuronsor Fra-Li ir along the subfornical organ, hypothalamic paraventricular nucleus (PVN,lateral mangocellular and medial parvocellular subnuclei) and supraoptic nucleus(anterior and medial sections) did not show any significant differences between thematernal treatments.Taking into account our previous evidence and the present results indicate that theavailability of a rich source of sodium during the pre/postnatal period induces a long-term effect on drinking and the basal neural activity along the OVLT implicated in thecontrol of hydroelectrolyte balance.