SACCHARIDE PATTERN IN GOAT PLACENTA DURING GESTATION

FLORES BRACAMONTE M C; GREGORI S; MURA N; DÍAZ T; ACUÑA F; BARBEITO C; BOZZO A

Mendoza

Otro; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biología de Cuyo

In the province of Córdoba, goat production is an economic source for farming families that develop this activity with limited technological resources. To optimize their productivity, it is important to guarantee an adequate weight of the offspring at birth. The growth and survivalof the fetus depends on the placenta, which is characterized by being cotyledonary and sinepitheliochorial with trophoblast gigant cells that migrate from the chorionic epithelium and fuse with the endometrial epithelium to form syncytia. Cell surface carbohydrates are involvedin the processes of cell recognition and adhesion between the trophoblast and the uterus. During placenta development, the composition of carbohydrates on the cell surface undergoes variations that have a fundamental role in the processes of cell recognition and adhesion between the trophoblast and the uterus. The placental pattern of carbohydrates has not been studied in this species. The aim of this study was to describe the pattern of carbohydrates in the goat placenta throughout gestation. Fifteen goats (Capra hircus) older than two years that had access to food and water ad libitum were used. They were sacrificed at 50, 100 and 135 days of gestation following the COEDI animal management recommendations Histological sections of placentas were obtained and the lectinhistochemistry technique was performed with biotinylated lectins: GSL-I, WGA, SJA, SWGA, PSA and LCA. The placental structures analyzed were glycocalyx (GC), binucleated cells (BN), mononucleated cells (MN), syncytia (S), and fetal (EF) and maternal (EM) endothelium. The intensity of binding to each lectin was qualitatively rated on a scale of 0 (negative), + (weak), ++ (moderate) to +++ (strong). 10 random fields were analyzed for each slice of placental tissue. At 50 days of gestation, the structures that possessed +++ binding intensity were the GC of mononucleartrophoblastic cells and EFs with GSL-I and EMs with WGA. At 100 days, the GC of mononuclear trophoblastic cells showed +++ binding intensity with GSL-I, the EF had +++ binding intensity with GSLL-I and WGA, and the EM showed +++ binding intensity with WGA. At 135 days, +++ GC binding of mononuclear trophoblastic cells was identified with GSL-I and EM and EF showed +++ binding intensity with GSL-I, WGA and LSA. The pattern of placental carbohydrates allowed evidence of remodeling in the different gestational stages studied.