LIPID SIGNAL DURING THE INTERACTION Fusarium graminearum-Hordeum vulgare

REYNA, M; PEPPINO MARGUTTI, M; TORRES, A M; DARDANELLI, M S; RACAGNI, G E; STENGLEIN, S A; VILLASUSO, A

La Falda

Congreso; XXI Jornadas de divulgaciones Científicas de la Sociedad de Biología de Córdoba; 2017

Sociedad de Biología de Córdoba

PLA2 activity is one of the signaling mechanisms involved in response to biotic stress. Its products, free fatty acids and lysophospholipids, are of great importance in plant-pathogen interaction. Species within the Fusarium graminearum complex produce fusariosis and mycotoxin contamination, reducing the amount and quality of grains in cereals. The objective of this work was evaluated lipid signaling involved in the interaction between Fusarium graminearum and barley roots. In principle, the contribution of PLA2 activity, with NBD-PC as substrate, and total fatty acid (GA) by GC / ME were evaluated. The interaction of barley seedlings with macroconidia of F. graminearum increased the activity of PLA2 rapidly and transiently (between 15 and 30 minutes of exposure to the pathogen). The similar but displaced response over time has been observed over the content of AG 18: 2 - 18: 3, which increased only after 30 min. This fact modified the relation AG nonsaturated / Saturated to all times times. Our results suggest that during interaction with Fusarium graminearum barley roots modulate PLA2 activity, and that the resulting unsaturated AGs, especially 18: 3, are targeted to the biosynthesis of phytohormones as JA.