LIPID SIGNALING DURING THE INTERACTION FUSARIUM GRAMINEARUM-HORDEUM VULGARE

REYNA, M; PEPPINO MARGUTTI, M; TORRES, A M; DARDANELLI, M S; RACAGNI, G E; STENGLEIN, S A; VILLASUSO, A L

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2017 vol. 42

0327-9545

Phospholipase A2 (PLA2) activity is one of the signaling mechanisms involved in the response to biotic stress. Its products, free fatty acids and lysophospholipids, are very important in plant-pathogen interaction. Species within the Fusarium graminearum complex produce fusariosis and mycotoxin contamination, reducing the amount and quality of grains in cereals. The objective of this work was to evaluate the lipid signaling involved in the interaction between Fusarium graminearum and barley roots. Initially, PLA2 activity using NBD-PC as the substrate and total free fatty acids (FFA) by GC / ME were evaluated. The interaction of barley seedlings with macroconidia of F. graminearum increased the activity of PLA2 rapidly and transiently (between 15 and 30 minutes of exposure to the pathogen). A similar response, although shifted in time, was observed on the content of FFA 18:2 - 18:3, which increased only after 30 min. This fact modified the unsaturated/saturated FFA ratio at all the times evaluated. Our results suggest that the interaction of F. graminearum, with barley roots modulates PLA2 activity and that the resulting unsaturated FFAs, especially 18:3, are targeted to the biosynthesis of phytohormones as JA.