Analysis of the ATP/CD39 axis in pediatric RSV infection

RUSSO, CONSTANZA; RAIDEN, SILVINA; ALGIERI, SILVIA; DE CARLI, NORBERTO; SARLI, MARIAM; BRUERA, MARÍA J.; CAIROLI, HÉCTOR; GONZALEZ, FERNANDA; DE LILLO, LEONARDO; SEERY, VANESA; SANANEZ, INÉS; SIMAZ, NANCY; FERRERO, FERNANDO; GEFFNER, JORGE; ARRUVITO, LOURDES

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC-SAI-FAIC-SAFIS; 2022

SAIC-SAI-FAIC-SAFIS

Background: Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis in young children. Extracellular ATP founded in inflammatory environments is regulated by CD39. There is no data regarding the role of purinergic signaling in the lung inflammation during RSV infection. Aims: 1) to quantify ATP levels in serum, nasopharyngeal aspirates (NPA) and the supernatants of stimulated PBMCs; 2) to analyze the frequency and phenotype of CD4+CD39+ cells in peripheral blood; 3) to explore the biologic response of CD4+ T cells upon incubation with an antagonist of CD39. Methods: Plasma, NPA and PBMCs from children with non-severe (n=46) and severe RSV infection (n=25) and controls (n=17) were used. Results: We found higher levels of ATP in plasma from RSV children, compared with controls (p