BECAS
PARRA Leandro GastÓn
congresos y reuniones científicas
Título:
Effect of fatostatin on hyperosmolality-activated lipid metabolism in renal epithelial cells.
Autor/es:
CASALI, CI; WEBER, K; MESSINGER, D; PARRA, L; FERNÁNDEZ-TOME, MC
Lugar:
Buenos Aires
Reunión:
Congreso; XLIX Reunión de la Sociedad Argentina de Investigaciones Bioquimicas y Biología Molecular; 2013
Institución organizadora:
SAIB
Resumen:
In the renal cell line MDCK, we demonstrated that the increase in
environmental osmolarity up-regulates phospholipid (PL) and triglycerides (TG)
metabolism. The aim of the present work was to evaluate whether or not the
increase in lipid metabolism requires the activation of the transcription
factor sterol response element binding protein (SREBP). With this purpose, MDCK
cells were incubated in isosmolar and hyperosmolar medium (containing 125 mM
NaCl) alone or in the presence of different concentrations (10-25 mM) of fatostatin (FATO), a SREBP inhibitor. To evaluate lipid
synthesis 0.4 mCi [14C(U)]-glycerol was added to the incubation medium 3 h before cell
collection. After 24 h of treatment with NaCl, cells were collected, counted
and lipid content and biosynthesis determined. FATO decreased the number of
cells. Hypertonicity increased total PL and TG content by 60 and 100 %,
respectively, unexpectedly FATO increased PL and TG accumulation. FATO (10, 15
and 20 mM) decreased 14C-gly
incorporation into DAG (by 60, 70 and 80 %) but not change on PL and TG synthesis
was observed. These results suggest that hypertonicity would activate SREBP
which in turn would modulate the up-regulation observed in lipid synthesis
under hyperosmotic conditions.