Identification of soluble components in human adipose tissue explants from renal cell carcinoma.

CAMPO VERDE ARBOCCÓ F; JOSE D ROMEO; LEONARDO GIORLANDO ; NEOLIA LOPEZ; LOPEZ FONTANA G; LOPEZ FONTANA R; SANTIANO FE; LOPEZ FONTANA CM; SASSO CV; ZYLA L; LOPEZ FONTANA CM; CARON RW; PISTONE CREYDT VIRGINIA

Mendoza

Congreso; XXXIV Reunion Anual de la Sociedad de Biología de Cuyo; 2016

We have recently demonstrated that conditioned media (CMs) from human adipose tissue explants from renal cell carcinoma near the tumor (hRATnT) regulate adhesion and migration of tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells, contrary to CMs from human adipose tissue explants from renal cell carcinoma farther away from the tumor (hRATfT). Now, we have begun to identify soluble components present: 1) in hRATnT- and hRATfT-CMs by WB; 2) in tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells incubated with different CMs. We employed the CMs after 24 h of incubation of hRATnT and hRATfT. We evaluated the expression of versican, ADAMTS1, and adiponectin in the CMs. We incubated 786-O, ACHN and NK-2 cells with theCMs for 24 h, lisated the cells and evaluated the expression of AdipoR1, AdipoR2, ObR and leptin. We observed a higher expression of both versican and adiponectin, as well as a lower expression of ADAMTS1 in hRATnT-CMs compared to the expression in hRATfT-CMs (p