INVESTIGADORES
TRONO Karina Gabriela
artículos
Título:
An efficient vaccine agaisnt bovine leukemia virus
Autor/es:
GERONIMO GUTIERREZ; SABRINA RODRIGUEZ; AGUSTIN VILOR; GILLET NICOLAS; DE BROGNIEZ ALIX; KARINA TRONO; WILLEMS LUC
Revista:
RETROVIROLOGY
Editorial:
BIOMED CENTRAL LTD
Referencias:
Lugar: Londres; Año: 2015 vol. 12 p. 3 - 3
ISSN:
1742-4690
Resumen:
Previous attempts to produce a vaccine against BLV
faced problems of efficacy (i.e. only a fraction of animals
were protected), persistence (i.e. rapid decrease of
immune protection), cost (e.g. production of purified
proteins) or safety (e.g. genetically modified hybrid
viruses). We have designed a novel strategy based on
the use of a live-attenuated BLV provirus. The rationale
behind this strategy relies on the deletion of genes
required to induce pathogenesis maintaining integrity of
those involved in infectivity. We have identified a BLV
deleted provirus that is infectious in cattle but replicates
at reduced levels in cows as shown by real-time quantitative
PCR. The deletant elicits a strong anti-BLV
immune response as indicated by wild-type antibody
titers. Vaccinated animals but not uninfected controls
resist challenge by a wild type BLV virus. The deletant
does not spread to uninfected sentinels maintained during
5 years in the same herd supporting biosafety of the
vaccine. Passive immunity, but not viral infection, is
transmitted to the newborn calves via the maternal
colostrum. Assays regarding production, storage and
delivery of the vaccine, as well as safety of the milk produced
by vaccinated cows are currently being carried
out with the aim to start a large scale trial that will be
held in Argentina in real dairy conditions during this