A recombinan attenuated candidate vaccine that efficiently and persistently proects against bovine leukemia virus in herds

RODRIGUEZ SABRINA; GUTIERREZ GERONIMO; GILLET NICOLAS; TRONO KARINA; WILLEMS LUC

RETROVIROLOGY

BIOMED CENTRAL LTD

Lugar: Londres; Año: 2014 vol. 11

1742-4690

There currently is no efficient vaccine that protects against bovine leukemia virus (BLV) infection. We now propose a novel approach based on the use of a recombinantlive-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce pathogenesis maintaining the integrity of those involved in infectivity. We have identified a BLV attenuated provirus that is infectious but replicates at reduced levels in cattle. The attenuated strain elicits a strong antiBLV immune response and does not spread to uninfected sentinels maintained during 3 years in the same herdsupporting biosafety of the vaccine. Passive antibodies are transmitted to newborn calves via maternal colostrum and persist during several months. Nevertheless, the BLV attenuated provirus does not transmit from cows to calves. Finally, vaccinated animals resist challengeby a wild-type BLV virus. In summary, we have identified a safe BLV attenuated provirus with impaired transmissibility that efficiently protects against infection in herd conditions.