INVESTIGADORES
CECCHINI Nicolas Miguel
artículos
Título:
Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
Autor/es:
ROYCHOUDHRY, SURUCHI; GREENBERG, JEAN T.; CECCHINI, NICOLÁS M.
Revista:
STAR Protocols
Editorial:
Cell Press - Elsevier
Referencias:
Año: 2024 vol. 5
ISSN:
2666-1667
Resumen:
Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.
