PIEZO-ICSI FERTILIZATION IN BOVINE OOCYTES

GABRIEL MARTIN ALVAREZ; ELIZABETH BREININGER; PABLO DANIEL CETICA

Jornada; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; 2020

SAB

ICSI is inefficient technique in bovine embryo production and chemical activation of oocytes is mandatory. The aims were to evaluate the bovine embryo production efficiency by Piezo-ICSI and to compare the PLC activity in bull and human sperm. In vitro matured bovine oocytes were fertilized by conventional ICSI and Piezo-ICSI. Number of pronuclei, embryo cleavage and sperm PLC enzymatic activity were determined by fluorometry. Oocyte survival (99 vs 95 %), 2 pronuclei oocytes (22,3 vs 5,9 %) and embryo cleavage rates (18,4 vs 4,2 %) were higher when Piezo-ICSI was performed (P