PIEZO-ICSI AS ALTERNATIVE TOOL TO IMPROVE OOCYTE ACTIVATION IN IN VITRO MATURED BOVINE OOCYTES MODEL

GABRIEL MARTIN ALVAREZ; SOFIA VILLANUEVA; MARISA GELLER; PABLO DANIEL CETICA; GABRIEL CARLOS DALVIT

Denver

Congreso; The 74th Scientific Congress, American Society for Reproductive Medicine (ASRM); 2018

American Society for Reproductive Medicine (ASRM)

ObjectiveEvaluate the fertilization rates in a bovine oocyte activation failure model of Piezo-ICSI compared to conventional ICSIDesignWe aimed to evaluate the participation of Piezo-pulse and sperm species in oocyte activation. In vitro matured bovine oocytes were randomly allocated to one of four groups: 1-conventional ICSI with bull sperm, 2- Piezo-ICSI with bull sperm, 3- conventional ICSI with human sperm, 4- Piezo-ICSI with human sperm.Materials and methodsCumulus-oocyte complexes were obtained from bovine ovaries from slaughterhouse and matured in vitro. Cryopreserved sperm from bulls and humans with proven fertility were used. Conventional ICSI was performed with bevelled spiked micropipettes. Piezo-ICSI was performed with flat-tipped micropipettes. Fertilization rates were evaluated 18 h after ICSI by fixing and staining the presumptive zygotes with Hoechst 33342. Cleavage rates were evaluated 48 h after ICSI.Results Fertilization rate (2 pn) was higher with Piezo-ICSI (22.3%) than conventional ICSI (5.9%) using bull sperm (P