GLYCOLYSIS, PENTOSE PHOSPHATE PATHWAY AND MEIOTIC PROGRESSION OF PORCINE OOCYTES DURING IN VITRO MATURATION

GABRIEL MARTIN ALVAREZ; SEBASTIÁN CASIRÓ; SERGIO ADRIÁN MORADO; GABRIEL CARLOS DALVIT; PABLO DANIEL CETICA

Chascomus

Jornada; XV Jornadas Anuales de la Sociedad Argentina de Biología; 2013

Sociedad Argentina de Biología

We have previously observed the inhibition of glycolysis by sodium fluoride (NaF) and ATP and the inhibition of the pentose phosphate pathway by 6-aminonicotinamide (6-AN) and NADPH in porcine cumulus-oocytes complexes (COCs). Our aim was to determine the influence of these inhibitors on the meiotic progression of porcine oocytes during in vitro maturation. COCs were cultured during 44 h at 39ºC in medium 199 with gonadotropins (control) + 5 mM NaF, 10 mM ATP, 0.025 mM 6-AN or 0.125 mM NADPH. Oocyte nuclear stage was determined at 0, 24, 32, 40 and 44 h of maturation by Hoechst 33342 stain. Inhibition of glycolysis by NaF or ATP decreased the percentage of oocytes reaching metaphase II (P