In vitro porcine cumulus expansion and oocyte maturation

GABRIEL MARTIN ALVAREZ; GABRIEL CARLOS DALVIT; MATIAS TELLADO; MARCELO SERGIO MIGUEZ; PABLO DANIEL CETICA

Huerta Grande, Córdoba, Argentina

Jornada; Primera Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

We have previously observed that cumulus-oocyte complex (COC) quality is related to both nuclear and cytoplamic maturation. The aim was to evaluate oocyte meiotic and cytoplasmic maturation according to cumulus expansion features. COCs recovered from slaughtered gilts were selected according to cumulus characteristics in classes: A1 complete-multilayer-dark and A2 complete-multilayer-light. COCs were matured in medium 199 for 48h and then classified in fully expanded, partially expanded or spontaneous partially denuded. Oocytes were used to evaluate nuclear maturation (metaphase II) or incubated in TBMm with fresh spermatozoa during 18h. Cytoplasmic maturation was evaluated by sperm head decondensation and/or pronuclear formation. In A1 and A2 no differences were found between the percentages of fully expanded and partially expanded COCs, but spontaneous partially denuded were lower (p