Regulación de la glucólisis en la maduración de ovocitos porcinos in vitro

GABRIEL MARTIN ALVAREZ; GABRIEL CARLOS DALVIT; PABLO DANIEL CETICA

Buenos Aires, Argentina

Jornada; X Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2008

Sociedad Argentina de Biología

We have previously demonstrated that glycolysis in cumulus-oocyte complexes (COCs) is stimulated by gonadotropins. The aim of the present study was to determine the effect of positive (AMP) and negative (ATP and sodium fluoride ?NaF-) regulators on glycolytic pathway and meiotic maturation of oocytes. COCs were aspirated from ovarian antral follicles (3-8mm) of slaughtered gilts and selected under stereomicroscope, only oocytes surrounded by a complete and dark cumulus were used. Oocyte maturation was performed for 48 h in 199 medium supplemented with FSH and LH (control), added with different concentrations of the regulators. Glucose and lactate concentration in the culture medium was measured by spectrophotometry. Meiotic maturation was evaluated by presence of metaphase II. There was no effect of AMP neither on glucose consumption and lactate production nor nuclear maturation at any concentration used. There was a dose dependent inhibition of glucose consumption, lactate production and meiotic maturation with both ATP and NaF (p