Producción in vitro de cigotos porcinos utilizando semen vitrificado

CLAUDIA ARRAZTOA; CLARA BACA CASTEX; GABRIEL MARTIN ALVAREZ; SUSANA GIULIANO; PABLO DANIEL CETICA; DEBORA NEILD

Congreso; IV Congreso Peruano de Reproducción Animal; 2014

Vitrification of porcine spermatozoa, using the spheres method in the absence of cryoprotectant, permits preservation of sperm chromatin condensation and integrity. It would be interesting to evaluate if vitrifiedwarmed porcine spermatozoa maintain the capacity to produce zygotes in vitro, using the ICSI technique. The results showed no significant differences in percentage of zygotes (P>0,05) between the group of oocytes injected with vitrified-warmed spermatozoa (42,9%; 39/91) and the group injected with extended semen preserved at 17° C (34,2%; 26/76). This shows that porcine sperm vitrification in spheres, using TALP medium without cryoprotectant, would not only preserve spermatozoa with condensed and intact chromatin, but also with the capacity to produce zygotes using the ICSI technique.