Pentose phosphate pathway control in porcine oocyte in vitro maturation

EUGENIA FERRETTI; GABRIEL MARTIN ALVAREZ; GABRIEL CARLOS DALVIT; PABLO DANIEL CETICA

San Juan

Jornada; II Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011

Instituto de Biología y Medicina Experimental (IBYME), Sociedad Argentina de Biología (SAB).

Glucose consumption by cumulus oocyte-complexes (COCs) during in vitro maturation (IVM) may in part be fated to pentose phosphate pathway (PPP). We previously observed a statistical association between PPP activity and oocyte nuclear in vitro maturation. The control of the pathway by pharmacological modulators will contribute to the understanding of PPP participation on oocyte maturation. The aim of this work was to evaluate PPP activity, oocyte nuclear maturation, and glucose uptake during IVM in the presence of an inhibitor of the dehydrogenases of the pathway (6-aminonicotinamide, 6-AN). COCs were recovered by aspiration of antral follicles of ovaries from slaughtered gilts and matured 48 h at 39ºC, 5% CO2 in medium 199 with gonadotropins (control) and with different concentrations of 6-AN (0.01, 0.025, 0.05 and 0.10 mM). PPP activity was evaluated by Brilliant Cresil Blue stain, glucose uptake was determined spectrophotometrically, and oocyte nuclear maturation by the presence of metaphase II. There were dose dependent decreases in PPP activity and in oocyte nuclear maturation rates (P