Inhibition of isocitrate dehydrogenase enzyme affects in vitro maturation of porcine oocytes

BRUNO VECCHI GALENDA; GABRIEL MARTIN ALVAREZ; PABLO DANIEL CETICA; ELIZABETH BREININGER

CABA

Jornada; XII Jornadas Anuales de la Sociedad Argentina de Biología; 2010

Instituto de Biología y Medicina Experimental (IBYME), Sociedad Argentina de Biología (SAB).

Maturation depends on the metabolic activity of the cumulus-oocyte complex (COC) that performs nutritive and regulatory functions during this process. However, there is little information about the metabolic profile of porcine COCs during in vitro maturation. Our aim was to determine the participation of isocitrate dehydrogenase enzyme in the in vitro maturation of porcine oocytes. COCs were recovered by aspiration of antral follicles (3-8mm) from ovaries of slaughtered gilts. In vitro maturation was performed during 48 h, 39ºC, 5% CO2 in medium 199 supplemented with gonadotropins and different concentrations (0-30 mM) of oxalomalate (inhibitor of isocitrate dehydrogenase). In vitro fertilization was performed in mTBM with fresh semen during 18 h. Nuclear and cytoplasmic maturation were evaluated by the presence of metaphase II chromosome configuration or decondensed sperm heads and pronuclei, respectively. The percentages were analized by the Chi-square test. Nuclear maturation diminished with 20 and 30 mM of oxalomalate (p