IMPLICATIONS OF GLYCOLYTIC AND PENTOSE PHOSPHATE PATHWAYS ON THE OXIDATIVE STATUS AND ACTIVE MITOCHONDRIA OF THE PORCINE OOCYTE DURING IN VITRO MATURATION

GABRIEL MARTIN ALVAREZ; SEBASTIÁN CASIRÓ; CYNTHIA GUTNISKY; GABRIEL CARLOS DALVIT; MELANIE SUTTON-MCDOWAL; JEREMY THOMPSON; PABLO DANIEL CETICA

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2016 vol. 86 p. 2096 - 2106

0093-691X

Glycolysis and the pentose phosphate pathway (PPP) were modulated in porcine cumulusoocytecomplexes during IVM by the addition of inhibitors and stimulators of key enzymesof the pathways to analyze their influence on the oxidative status, active mitochondria,and maturation of the oocyte. The influence of pharmacologic and physiological inhibitorsof glycolysis (Sodium fluoride and ATP) and PPP (6-Aminonicotinamide and nicotinamideadenine dinucleotide phosphate) was validated by assessing glucose and lactate turnoverand brilliant cresyl blue staining in oocytes. Inhibitors of glycolysis and PPP activitysignificantly perturbed nuclear maturation, oxidative metabolism (Redox Sensor Red CC-1), and active mitochondria (Mitotracker Green FM) within oocytes (P < 0.05). In comparison,physiological stimulators of glycolysis (adenosine monophosphate) and PPP(nicotinamide adenine dinucleotide phosphate) did not affect any of evaluated parameter.In the absence of modulators, fluctuations in the oocyte oxidative activity and activemitochondria were observed during porcine IVM. The inhibition of glycolysis and PPPmodified the pattern of oxidation and mitochondrial fluctuation, resulting in impairedmeiotic progression.We demonstrated the relationship between carbohydrate metabolismin COC and oocyte redox status necessary for porcine oocyte IVM.