INVESTIGADORES
ALVAREZ Gabriel MartÍn
artículos
Título:
The control of reactive oxygen species influences porcine oocyte in vitro maturation
Autor/es:
GABRIEL MARTIN ALVAREZ; SERGIO ADRIÁN MORADO; MARIELA SOTO; GABRIEL CARLOS DALVIT; PABLO DANIEL CETICA
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2015 vol. 50 p. 200 - 205
ISSN:
0936-6768
Resumen:
The aim of this study was to examine the effect of varying
intracellular reactive oxygen species (ROS) levels during
oocyte in vitro maturation with enzymatic ROS production
systems (xanthine + xanthine oxidase or xanthine + xanthine
oxidase + catalase), scavenger systems (catalase or superoxide
dismutase + catalase) or cysteine on porcine oocyte maturation.
Oocyte ROS levels showed an increase when H2O2 or
O2
- production systems were added to the culture medium
(p < 0.05). On the other hand, the presence of ROS scavengers
in the maturation medium did not modify oocyte ROS
levels compared with the control after 48 h of maturation,
but the addition of cysteine induced a decrease in oocyte ROS
levels (p < 0.05). The ROS production systems used in this
work did not modified the percentage of oocyte nuclear
maturation, but increased the decondensation of sperm head
(p < 0.05) and decreased the pronuclear formation (p < 0.05).
In turn, the addition of O2
- and H2O2 scavenging systems
during in vitro maturation did not modify the percentage of
oocytes reaching metaphase II nor the oocytes with decondensed
sperm head or pronuclei after fertilization. However,
both parameters increased in the presence of cysteine
(p < 0.05). The exogenous generation of O2
- and H2O2
during oocyte in vitro maturation would not affect nuclear
maturation or later sperm penetration, but most of the
spermatozoa cannot progress to form the pronuclei after
fusion with the oocyte. The decrease in endogenous ROS
levels by the addition of cysteine would improve pronuclear
formation after sperm penetration.