INVESTIGADORES
ALVAREZ Gabriel MartÍn
artículos
Título:
Modulation of glycolysis and the pentose phosphate pathway influences porcine oocyte in vitro maturation
Autor/es:
GABRIEL MARTIN ALVAREZ; EUGENIA FERRETTI; CYNTHIA GUTNISKY; GABRIEL CARLOS DALVIT; PABLO DANIEL CETICA
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 48 p. 545 - 553
ISSN:
0936-6768
Resumen:
Glycolytic and pentose phosphate pathway (PPP) activities
were modulated in porcine cumulusoocyte complexes
(COCs) during in vitro maturation (IVM) by the addition
of inhibitors or stimulators of key enzymes of the pathways
to elucidate their relative participation in oocyte maturation.
The activities of glycolysis and PPP were evaluated by lactate
production per COC and by the brilliant cresyl blue test,
respectively. Glucose uptake per COC and the oocyte
maturation rate were also evaluated. Lactate production,
glucose uptake and the percentage of oocytes reaching
metaphase II decreased in a dose-dependent manner in the
presence of the pharmacological (NaF) or the physiological
(ATP) inhibitors of glycolysis (p < 0.05). The addition of the
physiological stimulator of glycolysis (AMP) caused no effect
on lactate production, glucose uptake or the meiotic maturation
rate. The pharmacological (6-AN) and the physiological
(NADPH) inhibitors of PPP induced a dose-dependent
decrease in the percentage of oocytes with high PPP activity
and in the nuclear maturation rate (p < 0.05). The physiological
stimulator of PPP (NADP) caused no effect on the
percentage of oocytes with high PPP activity. The glycolytic
and PPP activities of porcine COCs and maturational
competence of oocytes seem to be closely related events.
This study shows for the first time the regulatory effect of
ATP and NADPH as physiological inhibitors of glycolysis
and PPP in porcine COCs, respectively. Besides, these
pathways seem to reach their maximum activities in porcine
COCs during IVM because no further increases were
achieved by the presence of AMP or NADP.