Endometrial expression of key genes related to fertility in repeat breeder and non-repeat breeder cows

JAUREGUIBERRY, MARÍA; MADOZ, LAURA VANINA; QUINTANA, SILVINA; MARÍN, MAIA; BURUCÚA, MERCEDES; TIZZANO, MARCO; RABAGLINO, BELÉN; REARTE, RAMIRO; PECORARO, MARCELO ÍTALO; DE LA SOTA, RODOLFO LUZBEL

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Año: 2020 vol. 55 p. 1660 - 1664

0936-6768

The aim of the present study was to compare the endometrial gene expression of epidermal growth factor receptor (EGFR), nodal growth differentiation factor (NODAL), prostaglandin-endoperoxide synthase 2 (PTGS2), oestrogen receptor 1 (ESR1) and progesterone receptor (PGR) in repeat breeder cows (RBC) and non-RBC during diestrus. Endometrial samples were collected by cytobrush technique and stored in RNA stabilizing solution at −20°C until RT-qPCR analysis. Differences in endometrial mRNA expression of selected genes were assessed by ANOVA and simple (r) and the partial correlations (rp) among selected genes were performed. Results demonstrated that mRNA expression of EGFR and NODAL were higher in RBC than in non-RBC (3 and 25-fold change, p