Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells

MATÍAS ANGEL SIRINI; JUAN PATRICIO ANCHORDOQUY; SILVINA QUINTANA ; CECILIA FURNUS; ALEJANDRO ENRIQUE RELLING, PH.D.1 , JUAN MATEO ANCHORDOQUY,

INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY

ROYAN INST

Año: 2018 vol. 12 p. 335 - 338

2008-076X

Energy balance is regulated by ghrelin which is a neuroendocrine modulator. Ghrelin is expressed in reproductive organs. However, the role of ghrelin during in vitro maturation (IVM) and bovine preimplantationaldevelopment is limited. The purpose of this study was to measure the expression of ghrelin (GHRL) andits receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA, and determine cumulus oocytecomplex (COC) viability after IVM with 0, 20, 40 and 60 pM of ghrelin. Also, pronuclear formation wasrecorded after in vitro fertilization (IVF). GHRL and GHS-R1A mRNA expression in oocyte and cumulus cells (CCs) was assessed using reverse transcription-polymerase chain reaction (PCR). Oocyte andCC viability were analyzed with the fluorescein diacetate fluorochrome-trypan blue technique. Pronuclearformation was determined 18 hours after IVF with Hoechst 33342. The results demonstrated that ghrelinmRNA is present in oocyte and CCs before and after 24 hours IVM with all treatments. Ghrelin receptor,GHS-R1A, was only detected in oocytes and CCs after 24 hours IVM with 20, 40 and 60 pM of ghrelin.Oocyte viability was not significantly different (P=0.77) among treatments. However, CC viability wassignificantly lower (P=0.04) when COCs were matured with ghrelin (77.65, 72.10, 66.32 and 46.86% for0, 20, 40, and 60 pM of ghrelin, respectively). The chance of two pronuclei forming were higher (P=0.03)when ghrelin was not be added to the IVM medium. We found that ghrelin negatively impacts CC viabilityand pronuclear formation