Leptospira Diagnosis in Serum and Urine of Dogs by Real Time PCR

M. RECAVARREN; S. QUINTANA; M. RIVERO; B. BALLESTEROS; E. SCIALFA

Journal of Veterinary Science & Medical Diagnosis

SciTechnol

Lugar: Henderson; Año: 2015 vol. 4

2325-9590

A real-time PCR assay to detect DNA of Leptospira interrogans serovars infecting 7 dogs from pure cultures and clinical samples was developed. DNA amplification from pure cultures 8 of serogroups Canicola, Pomona, Pyrogenes, Ballum and Icterohaemorrhagiae was performed by 9 real-time PCR showing an analytical sensitivity of 8 genomes. From 70 sera samples of suspected 10 canine leptospirosis, microscopic agglutination test (MAT) was performed to determine antibodies 11 and real-time PCR to detect leptospiral DNA, dog DNA internal control amplification was 12 performed in all samples to verify successful DNA extraction. Thirteen samples were positive by 13 real-time PCR and L-MAT ≤ 1/200 and ≥ 1/400 with co-agglutination which represents 7 and 10 14 days post infections. One urine sample was positive by real-time PCR which serum was prior 15 negative to MAT. The real-time PCR assay could successfully detect leptospiral DNA in clinical 16 samples from dogs in various stages of infection and can be used to complement MAT technique.