Analysis of salivary and serum fatty acids as markers of lipidic intake in humans

DEFAGÓ MD, REPOSSI G, VALENTICH MA, ACTIS AB

Buenos Aires

Jornada; X Jornadas Anuales de la Sociedad Argentina de Biología; 2008

Sociedad Argentina de Biología

Introduction: the lipidic intake is reflected in biological fluids. For instance, the serum cholesterol is frequently used as a marker of its dietary intake. No evidences about the correlation between dietary, salivary and serum fatty acids (FA) have been found. Purpose: to analyze the correlation between dietary, salivary and serum FA. Methodology: nine healthy subjects of both sexes who attended two hospitals of Córdoba, Argentina, in 2007 participated in the study. A validated quantitative-qualitative food frequency questionnaire was applied. The Interfood v.2 software gave information on FA of habitual intake (mg/day). Saliva samples were collected by the subjects according to the international procedure and those of blood by the lab personnel. The lipids were extracted by the Folch method and the FA methylesters were analyzed by gas chromatography (mg/ml saliva or serum). The Spearman correlation test was applied to study the association between dietary, salivary and serum FA. Results: a positive correlation (r>0 <1) between diet-saliva-serum for 4:0 and 14:0 FA was observed. A positive correlation was also found for 16:0, 18:0, 18:1 n-9 and 18:2 n-6 between diet-serum and for 20:4 n-6 between diet-saliva. Discussion: these preliminary results show a differential correlation between dietary-salivary and dietary-serum FA.