0030 Intestinal phase approach of Trichinella patagoniensis in balb/c mice

FARIÑA, FERNANDO ; PASQUALETTI, M; ERCOLE, M; BESSI, C; MONTALVO, F; VARGAS, CLAUDIA V.; KRIVOKAPICH SJ; RIBICICH MM

Conferencia; 15th International Conference on Trichinellosis; 2019

0030 Intestinal phase approach of Trichinella patagoniensisin balb/c miceF.A. Fariñaa,b*, M.I. Pasqualettia,b, M.E. Ercolea, C. Bessia,b, F. Montalvoa,C. Vargasb, S.J. Krivokapichc, M.M. Ribicicha,baUniversidad de Buenos Aires Facultad de Ciencias Veterinarias, Cátedra deParasitología y Enfermedades Parasitarias, CABA, ArgentinabCONICET ? Universidad de Buenos Aires Instituto de Investigaciones enProducción Animal (INPA), Buenos Aires, ArgentinacANLIS, Dr. Carlos G. Malbrán, Buenos Aires, Argentina*Presenting/corresponding author: fernandoaf@fvet.uba.arThe gut constitutes the initial place of contact of Trichinella with itshost. The duration of the parasitism and the number of NBL produced byfemales in the intestines determines the extension of the disease. The aim ofthe present research was to study different aspects concerning the intestinalphase of T. patagoniensis in BALB/c mice. 128 female BALB/c mice weredivided into two groups of 64 mice and were inoculated PO with 500 L1 larvaeof T. patagoniensis and 500 L1 larvae of T. spiralis respectively. Euthanasia Sci Parasitol 20, Special issue, August 2019ISSN 1582-136632was performed on days 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 18, 20 y 25 pi.Then, adult worm recovery from intestines were performed. Samples fromsmall intestine were cut and stained with hematoxylin and eosin. Systemiccytokine profile was evaluated during the intestinal phase of infection of T.patagoniensis from sera of animals euthanized on days 0, 2, 4, 6 and 9 p.i.using a mouse Th1/Th2/Th17 cytometric bead array kit (BD Biosciences, SanJose, CA). One‐way ANOVA was performed followed by the Bonferroni?smultiple comparison test. Intestinal adult worm recovery of T. patagoniensistook place until day 17. Intestinal adult worm recovery of T. spiralis took placeuntil day 25. The recovery of adult worms resulted statistically differentamong day 9 on and day 1 and among day 10 on and day 1 for the treatment T.spiralis and T. patagoniensis respectively (p-value < 0.05). Hyperemia, mucosaloedema and inflammatory infiltrate was observed from day 1 pi in both T.patagoniensis and T. spiralis infected animals while hyperplasia of Peyer?spatches was presented from day 20 pi. IFN-γ values progressively increaseafter the inoculation with Trichinella species until reaching a peak at 9 dpi inanimals infected with T. spiralis and T. patagoniensis. The concentration of IL10 reached a peak at 4 dpi in animals belonging to both experimental groups.The level of TNF showed a slight increased 3 dpi. IL-2, IL-4, IL-6 and IL-17showed no significant variation during the study period. Althoughhistopathologic changes of intestines and systemic cytokine profile shown asimilar pattern in both Trichinella species, the duration of the intestinal phaseevaluated as the time pi adult worms were able to remain in gut of BALB/cmice was shorter for T. patagoniensis than T. spiralis.The present research was approved by the Committee for the Use andCare of Laboratory Animals (CICUAL) of the Facultad de Ciencias Veterinarias,University of Buenos Aires, under permit number 2014/01.This work was supported by Universidad de Buenos Aires, Secretaríade Ciencia y Técnica Subsidio UBACyT 20020130100336BA and UBACyT220170200331BA and Ministerio de Ciencia y Tecnología, FONCyT SubsidioPICT-2015-2350 and PICT-2015-3469.