Response of water behavior at the interphase of phospholipid matrixes assesed by FTIR

ROSA ANTONIO SEBASTIÁN; DISALVO EDGARDO ANIBAL; FRIAS MARÍA DE LOS ANGELES

Buenos Aires

Workshop; STRUCTURE AND DYNAMICS OF GLASSY SUPERCOOLED AN NANOCONFINED FLUIDS; 2019

Lipid membranes are one of the most important biological matrixes in which biochemical processes take place. This particular lipidarrangement is driven by different water disposition interacting with it, which is related to different water states with different energylevels at this interphase.In our work, we report changes in water content and distinctive water states by FTIR when this self assembled matrix changes itsphysicochemical properties. The -OH stretching band in the liquid water IR spectrum is the principal region used to understand itsmolecular organization (4000-3000 cm-1). The strength of the hydrogen-bonding depends on the cooperative/anti-cooperative nature ofthe surrounding hydrogen bonds, with strongest hydrogen bonds giving the lowest vibrational frequencies. Thus, we can use water asmirror of the membrane state in this kind of biological systems.[1,2]Different phospholipids associate water at particular modes according to their structures; this may produce modulation of packing andhydration suitable for the incorporation of aminoacids, peptides and enzymes.[3]