INVESTIGADORES
DAMIANI Armando Mario
artículos
Título:
Development of a surveillance scheme for equine influenza in the UK and characterisation of viruses isolated in Europe, Dubai and the USA from 2010-2012?
Autor/es:
WOODWARD AL, RASH AS, BLINMAN D, BOWMAN S, CHAMBERS TM, DALY JM, DAMIANI A, JOSEPH S, LEWIS N, MCCAULEY JW, MEDCALF L, MUMFORD J, NEWTON JR, TIWARI A, BRYANT NA, ELTON DM
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 169 p. 113 - 127
ISSN:
0378-1135
Resumen:
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and
undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during
2010?2012, including in vaccinated animals, highlighting the importance of surveillance
and virus characterisation. Virus isolates were characterised from more than 20 outbreaks
over a 3-year period, including strains from the UK, Dubai, Germany and the USA. The
haemagglutinin-1 (HA1) sequence of all isolates was determined and compared with OIErecommended
vaccine strains. Viruses from Florida clades 1 and 2 showed continued
divergence from each other compared with 2009 isolates. The antigenic inter-relationships
among viruses were determined using a haemagglutination-inhibition (HI) assay
with ferret antisera and visualised using antigenic cartography. All European isolates
belonged to Florida clade 2, all those from the USA belonged to Florida clade 1. Two
subpopulations of clade 2 viruses were isolated, with either substitution A144V or I179V.
Isolates from Dubai, obtained from horses shipped from Uruguay, belonged to Florida
clade 1 and were similar to viruses isolated in the USA the previous year. The
neuraminidase (NA) sequence of representative strains from 2007 and 2009 to 2012 was
also determined and compared with that of earlier isolates dating back to 1963. Multiple
changes were observed at the amino acid level and clear distinctions could be made
between viruses belonging to Florida clade 1 and clade 2.