NEUTROPHIL AUTOPHAGY DURIING HUMAN ACTIVE TUBERCULOSIS IS MODULATED BY SLAMF1.

PELLEGRINI, JOAQUÍN MIGUEL; SABBIONE, FLORENCIA; MORELLI, MA. PAULA; MARTIN, CANDELA; TATEOSIAN, NANCY L.; CASTELLO, FLORENCIA A.; AMIANO, NICOLÁS O.; PALMERO, DOMINGO J.; LEVI, ALBERTO; COLOMBO, MARIA ISABEL; TREVANI, ANALIA SILVINA; GARCÍA, VERONICA E.

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología.; 2019

Sociedad Argentina de Inmunología.

BACKGROUND: Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominatein patient´s lungs. Neutrophilic infiltration represents a characteristic feature of severetuberculosis (TB) but neutrophil biology during TB remains poorly understood. Autophagy iscrucial for several neutrophil functions and can be modulated by different mediators. Thecostimulator SLAMF1 acts as a microbial sensor in macrophages but its expression inhuman neutrophils has not been evaluated.METHODS: We investigated whether SLAMF1 participates in neutrophil autophagy againstMtb. Neutrophils were isolated from heparinized blood from TB patients and healthy donorsand cultured (2x106 cells/ml) with Mtb-Ag (10µg/ml) ± SLAMF1 mAb (10µg/ml) during 2 h.Some experiments were performed in the presence of a NADPH-oxidase inhibitor. SLAMF1and autophagy levels were analyzed by flow cytometry and confocal microscopy. P valuesof