CO-ADMINISTRATION OF rNP INFLUENZA WITH NANOPARTICLES BY SUBCUTANEOUS ROUTE, INDUCE INTENSE HUMORAL AND CELLULAR IMMUNE RESPONSES IN BALB/C MICE

ABBA RL, ; GERMANO MJ; SCODELLER EA, ; CARGNELUTTI DE,; SÁNCHEZ MV

Mendoza

Otro; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Currently, the immune response induced by actual influenza vaccines is based on the induction of antibodies against surface viralproteins, which can neutralize the virus. These antibodies are specific of vaccine strains and do not protect against antigenicvariants or new subtypes. The induction of strong T cell responses to conserved internal viral proteins, as the viral nucleoprotein,is associated with reduced disease severity as well as broad cross-reactivity protection. In recent days, the evaluate the immuneresponses of a new vaccine based on recombinant influenza nucleoprotein (rNP), co-administered with non-porous silica oralumin oxide nanoparticles. To achieve this goal, groups of 5 female BALB/c mice were immunized subcutaneously with 2 dosesof 10 µg of rNP alone or co-administered with 250 µg of the different nanoparticles, on day 1 and 21. Mice were sacrificed onday 42, and sera and spleens were recollected. The humoral immune response was evaluated by determination of antigen specificIgG and IgG subtypes antibodies titers by ELISA. The cellular immune response was evaluated by determination of INF-γ and IL4 in supernatants of splenocytes re-stimulated with rNP after 72 h, by ELISA. The results showed a intense humoral immuneresponse by high IgG and IgG1subtype specific titers from mice immunized with rNP/silica (1/715680 and 1/6400 respectively)and rNP/alumin oxide (1/5440 and 1/500000 respectively) compared with rNP alone. A significant increase in production of INFγ but not IL-4, was elicited by splenocytes from mice immunized with rNP/silica and rNP/alumina (P < 0.05). The obtainedresults showed that the formulations of rNP/ silica as well as rNP/alumin oxide confer a Th1 bias response. In conclusion, the useof nanoparticles combinated with NP could be taken into account for the development of innovative vaccines against influenza