TNF RECEPTOR DEFICIENCY DECREASES NITRIC OXIDE PRODUCTION AND DECREASES LOCAL INFLAMMATION DURING CUTANEOUS LEISHMANIASIS

MACKERN-OBERTI JP; GERMANO MJ; SÁNCHEZ-QUIROGA AL; SÁNCHEZ MV; TONELLI RL; SALOMÓN MC; CARGNELUTTI DE,

Mendoza

Otro; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Leishmaniasis caused by Leishmania (Leishmania) amazonensis (LA) is a tropical infectious disease affecting different countriesin South America. Due to LA infects macrophages, development of a Th1 immune response with production of TNF-α and IFN-γis essential to eliminate this pathogens. The aim of our work was to evaluate the role of TNF receptor p55 subunit (TNF-R) inIFN-γ-induced nitric oxide (NO) production by peritoneal macrophages and their impact in cutaneous leishmaniasis. To this endwe obtained peritoneal macrophages from TNF-R KO and WT mice and cultured for 24 hs with LPS (1µg/ml) and IFN-γ (10UI/ml). NO production was determined by the Griess Reaction. Cutaneous leishmaniasis were performed by the infection of1x105 LA promastigotes at mice left hinds pads. After 7 weeks, cutaneous lesions were surgery obtained, minced, stained withCD45 and analyzed by flow cytometry. We found that TNF-R KO macrophages showed a decreased production of NO afterLPS+IFNγ compared to WT (WT,untreated UT, 2,1±0,9µM;WT,LPS+IFNγ,19,0±1,5µM;TNF-R KO,UT 1,1±0,5 µM;TNF-RKO, LPS+IFNγ, 3,±0,5 µM; p