MEMBRANE COMPOSITION INFLUENCE ON THE HYPOTONIC HEMOLYSIS PROTECTION INDUCED BY ARGINE-BASED SURFACTANTS

HERMET, M.; FAIT, M.E.; VÁZQUEZ, R.; MATÉ, S.; HERLAX, V.; MORCELLE, S.R.; BAKÁS, L.

Tucumán

Congreso; IIILAFeBS, IX IberoAmerican Congress of Biophysics, XLV Reunion Anual SAB 2016.; 2016

Surfactants are one of the most widely used excipients in the pharmaceutical and cosmetic industries. In this way, amino acid-based surfactants constitute an important class of natural surface-active biomolecules with biocompatible properties and multifunctional activities.As surfactants are able to interact with the lipid bilayer of cell membranes, their surface and interface activities in systems involving interaction with membranes have been the subject of intense study. Because of the simplicity of the erythrocyte structure, red blood cells are one of the most popular cell membrane systems to study the surfactant-membrane interaction as well as the mechanisms underlying surfactant-induced osmotic cell resistance.Bz-Arg-NHC10 and Bz-Arg-NHC12 are two novel arginine-based surfactants synthesized in our laboratory. In order to clarify their lytic mechanism and the possible relation between the surfactant?s effect and the membrane?s biophysical properties, we employed mammalian red blood cells of two different species as models. Human and sheep red blood cells (HRBC and SRBC respectively) were chosen due to their differences in membrane protein/lipid composition.