BECAS
RODRÍGUEZ VARELA Maria Soledad
capítulos de libros
Título:
Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions
Autor/es:
ISAJA, LUCIANA; FERRIOL-LAFFOUILLERE, SOFÍA LUJÁN; MUCCI, SOFÍA; RODRIGUEZ VARELA, MARÍA S.; ROMORINI, LEONARDO
Libro:
Methods in Molecular Biology
Editorial:
Springer
Referencias:
Lugar: Nueva York; Año: 2021;
Resumen:
Human embryonic stem cells (hESCs) can differentiate into any cell lineage(pluripotency potential) derived from the three germ layers: ectoderm,mesoderm, and endoderm. Pluripotency is usually demonstrated in vitroby spontaneous differentiation of hESCs grown on a monolayer of feedercells using an embryoid bodies (EBs)-based method. However, currentlyhESCs are grown mostly using fully defined media in the absence of afeeder layer. Here we describe a EBs-based protocol that allowsmultilineage differentiation of hESCs and human induced pluripotent stemcells (hiPSCs) grown on feeder-free conditions.
