BECAS
ORTELLADO Laura Ester
capítulos de libros
Título:
Two-Dimensional Gel Electrophoresis: Discovering Isoenzymes for Mycoremediation
Autor/es:
SADAÑOSKI MARCELA ALEJANDRA; ORTELLADO LAURA ESTER; FONSECA MARÍA ISABEL; BENÍTEZ SILVANA FLORENCIA; ZAPATA PEDRO DARÍO
Libro:
Mycoremediation Protocols
Editorial:
Springer-Verlag New York Inc.
Referencias:
Lugar: New York, NY, United States; Año: 2022;
Resumen:
Exploring catabolic pathways and the enzymes involved in these processes allows enhancing mycoremediation treatments. Laccase and lipolytic enzymes are involved in the degradation of various pollutants. In this chapter, we present a methodology that combines SDS-PAGE and ND-PAGE to identify laccases and lipolytic enzymes and their isoenzymes. This technique not only provides information on molecular weight or the existence of isoenzymes but also provides a preliminary and simple way to determine the affinity for short- or long-chain fatty acids, using fluorescent substrates.