MATERNAL STRESS CAUSES ALTERATIONS IN NEONATAL IMMUNE RESPONSE

ALDIRICO MA; RATTAY G; PERRONE SIBILIA MD; SOTO A.; MORETTA R; GOLDMAN A; FENOY IM

San MIguel de Tucumán

Congreso; LXVII Reunión de la Sociedad Argentina de Inmunología; 2019

RATIONALE: We have shown that maternal stress during pregnancy results in an increase of litter susceptibility to develop allergic lung inflammation. In this work, we aimed to study the mechanisms involved in this phenomenon. METHODS: Pregnant BALB/c mice were subjected to restraint stress on days 15th and 17th of pregnancy. On day 5 after birth, lymphoproliferation was evaluated in pup?s splenocytes from stressed dams by 3H-thymidine incorporation. We also performed co-culture of pups splenocytes with purified CD4+ from DO11.10 mice along with OVA to measure IL-5, IL-4 and IFN-ƴ levels by ELISA. The remaining pups were sensitized intraperitoneally with OVA/Alum. At 0, 3, 6 and 18 hours after i.p. injection, we assessed cytokine levels in peritoneal lavage fluid by ELISA. Negative control group included pups from non-stress dams subjected to each of the protocols. RESULTS: Splenocytes from Stress group showed higher proliferation levels compared to splenocytes from Control group(p