INVESTIGADORES
MORETTA Rosalia Ester
congresos y reuniones científicas
Título:
EXPLOITING Anaplasma marginale GENOME
Autor/es:
MORETTA R.; RUYBAL P.; PETRIGH R.; WILKOWSKY S.; FARBER M.
Lugar:
Iguazú, Misiones
Reunión:
Congreso; XL Reunión Anual SOCIEDAD ARGENTINA DE INVESTIGACIÓN BIOQUÍMICA Y BIOLOGÍA MOLECULAR; 2004
Institución organizadora:
SOCIEDAD ARGENTINA DE INVESTIGACIÓN BIOQUÍMICA Y BIOLOGÍA MOLECULAR
Resumen:
The obligate intraerythrocytic bacterium Anaplasma marginale
(Rickettsiales: Anaplasmataceae) is the most prevalent tick-borne
livestock pathogen worldwide and is a severe constraint to animal
health and production in tropic and subtropical regions of the world.
Whole -genome sequencing of bacteria together with in silico
analysis and different kinds of genomic approaches are powerful
tools that allow prediction of genes that take part in microbial
pathogenesis. Using the information that became available with
the complete sequence of A. marginale, our study aimed at selecting
approaches that led us to the identification of genes that code for
putative virulence factors or immunogens. The selected methods
were:- PhoA fusion system for identifying exported proteins;- PCRbased
subtractive hybridization for detecting differential genes
between two species;-Bioinformatics analysis. We studied eight
gene products so far: five were identified using the PhoA fusion
system, two were selected from the differential subtraction library
and one was identified by bioinformatics analysis. We also found
a polymorphic tandem repeats of 11 nucleotides located in a noncoding
region of the genome. Among the selected genes, four are
currently being evaluated to determine their antigenic potential
and one of them is being characterized to elucidate its role as a
virulence determinant. In addition, the tandem-repeat non-coding
region is being validated as a diagnostic and epidemiological tool.