Customized designs of immobilized biocatalysts over no conventional magnetic nanoparticles for use in sustainble technologies

CITNIA M. ROMERO; CAROLINA NAVARRO; ANDRÉS H. MORALES; FLORENCIA CECILIA SPUCHES; MARIA A. MARTINEZ; MARÍA I. GÓMEZ

Montevideo

Simposio; Simposio Latinoamericano de Biocatálisis y Biotransformaciones; 2016

Universidad de la República y Universidad ORT Uruguay

Enzymes have extensive applications in food industries. Some of the major class of industries such as health care, pharmaceuticals and chemical manufacturing have been increased due to the catalytic nature of enzymes. Past few years have marked the significance of enzymes in production of biofuels such as lipases for biodiesel or glycocyl hydrolases (cellulases, xylanases) for bioetanol. However, all these desirable characteristics of enzymes and their widespread industrial applications are often obstructing by their lack of long-term operational stability, shelf life and by their recovery and reusability. Enzyme immobilization is one of the strategies to overcome these problems. Nanoparticles act as very efficient support materials for enzyme immobilization, because of their ideal, including specific surface area, mass transfer resistance, and effective enzyme loading. In addition, magnetic nanoparticles possess additional advantage, can be separated easily using an external magnetic field. Studies have shown that immobilization of enzymes to the nanoparticles can reduce protein unfolding and can improve stability and performance.