Anti-inflammatory effect of archaeolipid nanoparticles on human endothelial cells.

CHARÓ, NANCY LORENA; JEREZ, HORACIO EMANUEL; TATTI, SILVIO; ROMERO, EDER LILIA; SCHATTNER, MIRTA ANA

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

Background: New generation of liposomes called archaeosomes (ARC) are nanoparticles (NPs) made of archaeolipids employed as drug delivery systems that exhibit high structural stability. So far there are no reports on the effects of ARC on human vascular endothelium, critical data for their eventual clinical use.Aim: The present study investigated the effects of archaeosomes on human umbilical vein endothelial cells (HUVECs) in physiological and under inflammatory conditions and compared them with con- ventional liposomes.Methods: HUVECs were non-stimulated or stimulated with LPS, Pam3CSK4, E. coli, TNF-α, or IL-1 β in the absence or presence of nanoparticles. We analyzed cell viability and apoptosis (acridine orange/ethidium bromide), cell proliferation (EdU assay), expression of ICAM-1 and E-selectin (cytometry), secretion of IL-6 and von Wil- lebrand Factor (vWF) (ELISA), mRNA expression of ICAM-1 and vWF (RT-qPCR), uptake of NPs (cytometry and confocal microsco- py) and signaling pathways (cytometry and western blot). Results were analyzed by ANOVA followed by Bonferroni test (p