The anti-inflammatory effect of nanoarchaeosomes on human endothelial cells

CHARÓ, NANCY LORENA; JEREZ, HORACIO EMANUEL; TATTI, SILVIO; ROMERO, EDER LILIA; SCHATTNER, MIRTA ANA

Pharmaceutics

Multidisciplinary Digital Publishing Institute (MDPI)

Lugar: Basilea; Año: 2022

1999-4923

Archaebacterias are acknowledged as a unique source of novel biomaterials of interest in nano-medicine. In such a frame, the effects of nanoarchaeosomes (ARC), nanovesicles prepared from polar lipids extracted from the extreme halophilic Halorubrum tebenquinchense, were deter-mined on human umbilical vein endothelial cells (HUVEC) in physiological and under inflam-matory static conditions. Upon incubation, ARC (170 nm mean size, -41 mV ζ) did not affect the viability, cell proliferation, and expression of ICAM-1 and E-selectin under basal conditions, but reduced the expression of both molecules and the secretion of IL-6 induced by LPS, Pam3CSK4 or E.coli. Such effects were not observed upon TNF-α or IL-1β stimulation. Interestingly, ARC sig-nificantly decreased basal von Willebrand factor (vWF) levels and those induced by all stimuli. None of these parameters were modified by liposomes made of hydrogenated phosphatidylcho-line and cholesterol of comparable size and concentration. Only ARC were endocytosed by HU-VECs and reduced mRNA expression of ICAM-1 and vWF via NF-kB and ERK1/2 in LPS-stimulated cells. This is the first report on the anti-inflammatory effect of ARC on endothelial cells. Loaded with anti-inflammatory drugs, ARC could magnify their activity on inflamed en-dothelium, and therefore their research in vasculopathies is of special interest.