BEHAVIOR OF DIFFERENT CELL LINES IN CONTACT WITH SURFACES BASED ON POLY-N-ISOPROPYLACRYLAMIDE

CAPELLA, V.; RIVERO, R.E; LIAUDAT, A.C.; ROMA, D. A.; MAÑAS, F.; BARBERO, C.A.; BOSCH, P.; RIVAROLA, C.R; RODRIGUEZ, N.

Ciudad Autónoma de Buenos Aires

Jornada; XX Jornadas Anuales de la Sociedad Argentina de Biología (SAB)-XVII Jornadas de la Sociedad Uruguaya de Biociencias (SUB). Segundas Jornadas Rioplatenses de Biología; 2018

Sociedad Argentina de Biología (SAB) y la Sociedad Uruguaya de Biociencias (SUB)

In the field of tissue engineering, great advances have been generated in order to create biomaterials that function as scaffolds for cell growth. Several hydrogels simulate the mechanical properties of extracellular matrix (ECM), due to its innate similarity in structure and composition, providing a platform that mimic the native cellular milieus and allow a correct growth of several cells. Hydrogel based on N-isopropylacrylamide (PNIPAM) is one of the most studied materials in biomedical field; however more extensive biocompatibility and cellular interactions studies with different cell lines are needed to corroborate its biocompatibility. Therefore, the aim of this study is evaluate the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, proliferation and cell adhesion tests in murine pre-adipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549). MTT and neutral red uptake assays shown noncytotoxic effect of PNIPAM in any of the studied cell lines. Genotoxicity was evaluated by Comet Assay, where DNA damages were not detected. [3H]-thymidine staining allowed corroborating that cell cycle had progressed normally, allowing a correct proliferation. Adopted morphologies for each cell line over PNIPAM indicate that the surfaces favor the cell attachment during five days culture. The good biocompatibility of PNIPAM surface makes it a potential 2D scaffold for a possible adipose and kidney tissue-engineered construct.