PERSONAL DE APOYO
CACCIAMANI Valeria
artículos
Título:
CAVEOLIN-1 AND HSP70 INTERACTION IN MICRODISSECTED PROXIMAL TUBULES FROM SPONTANEOUSLY HYPERTENSIVE RATS (SHR) AS AN EFFECT OF LOSARTAN
Autor/es:
MANUCHA WALTER; BOCANEGRA VICTORIA; MARCELO RODRIGUEZ PEÑA; CACCIAMANI VALERIA; GARRAMUÑO VALLÉS, PATRICIA
Revista:
JOURNAL OF HYPERTENSION
Editorial:
LIPPINCOTT WILLIAMS & WILKINS
Referencias:
Lugar: Philadelphia; Año: 2010 p. 143 - 155
ISSN:
0263-6352
Resumen:
Abstract: Caveolin is required to traffic the AT1 receptor through the exocytic pathway. The chaperoneHsp70 regulates a diverse set of signaling pathways via their interactions with proteins. Here weexamined the AT1 receptor antagonist Losartan effect on Caveolin-1 and Hsp70 protein association inSHR proximal tubules (PT). Hsp70 involvement in Losartan oxidative stress regulation, was alsostudied. Five-week-old SHR were randomized for receiving Losartan (40 mg.kg-1day) (SHRLos) or notreatment (SHRH2O) during 6 weeks. Wistar-Kyoto rats (WKY) were normotensive controls. Bywestern blotting, the relative abundance of Caveolin-1 was 2-fold higher in microdissected PTmembrane fractions from treated SHR vs WKYH2O. Hsp70 membrane translocation was demonstratedin SHRLos through out the upregulation of Hsp70 expression in microdissected PT membrane fractionswhen compared with WKYH2O, p
