PERSONAL DE APOYO
KOLKOWSKI Edgardo
artículos
Título:
Pattern of cytokine receptors expressed by human dendritic cells migrated from dermal explants.
Autor/es:
LARREGINA A; MORELLI A; KOLKOWSKI EC; SANJUAN N; BARBOZA ME; FAINBOIM L
Revista:
IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 1997 vol. 91 p. 303 - 313
ISSN:
0019-2805
Resumen:
Different reasons account for the lack of information about the
expression of cytokine receptors on human dendritic cells (DC): (a) DC
are a trace population; (b) the proteolytic treatment used to isolate DC
may alter enzyme-sensitive epitopes; and (c) low numbers of receptors
per cell. In the present work the expression of cytokine receptors was
analysed by flow cytometry on the population of dermal DC (DDC) that
spontaneously migrate from short-term culture dermal explants. DDC
obtained after dermal culture were CD1alow, CD1b+, CD1c+, human
leucocyte antigen (HLA)-DR+, CD11chigh, CD11b+ and CD32+. The DC lineage
was confirmed by ultrastructural analysis. DDC expressed interleukin
(IL)-1R type 1 (monoclonal antibody (mAb) hIL-1R1-M1; and 6B5); IL-1R
type 2 (mAb hIL-1R2-M22); IL-2R alpha chain (mAb anti-Tac; and
hIL-2R-M1) and IL-2R gamma chain (mAb 3B5; and AG14C). DDC did not stain
for IL-2R beta chain using four mAbs recognizing two different epitopes
of IL-2R beta (mAb 2R-B; Mik-beta 1; and CF1; Mik-beta 3,
respectively). DDC were also positive for the cytokine binding chains
(alpha chains) of IL-3R (mAb 9F5); IL-4R (mAb hIL-4R-M57; and S456C9);
and IL-7R (mAb hIL-7R-M20; and R3434). DDC showed low levels of IL-6R
alpha chain (mAb B-F19; B-R6; and B-E23) and its signal transducer gp130
(mAb A2; and B1). DDC strongly expressed interferon-gamma receptor
(IFN-gamma R) (mAb GIR-208) and were negative for IL-8R (mAb B-G20; and
B-F25). All DDC were highly positive for granulocyte-macrophage
colony-stimulating factor receptor (GM-CSFR) alpha chain (mAb
hGM-CSFR-M1; SC06; SC04, and 8G6) and to a lesser extent for the common
beta chain of GM-CSFR, IL-3R and IL-5R (mAb 3D7). On the other hand,
reactivity was not found for granulocyte colony-stimulating factor
receptor (G-CSFR) (mAb hGCSFR-M1) nor macrophage colony-stimulating
factor receptor (M-CSFR) (mAb 7-7A3-17) confirming the DC lineage of
DDC. As previously reported for lymphoid DC, DDC expressed tumour
necrosis factor receptort (TNFR) 75000 MW (mAb utr-1; hTNFR-M1; and
MR2-1) but lacked TNFR 55000 MW (mAb htr-9; MR1-1; and MR1-2). In
summary, DDC express receptors for a broad panel of cytokines, even
receptors for cytokines whose effects on DC are still unknown (i.e.
IL-2R alpha gamma; IL-6R alpha/gp 130; IL-7R alpha gamma).