Allopregnanolone induces proliferation and migration of human ovarian cell lines IGROV-1 and SKOV-3

SANHUEZA MARÍA ÁNGELES; PELEGRINA, LAURA T; ANTONELLA R. R. CÁCERES; RODRIGUEZ, CRISTINA E; LACONI MYRIAM

MENDOZA

Congreso; XXXIV REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2016

SBC

Ovarian carcinoma is one of the most common fatal gynecologic malignancies in the world. Allopregnanolone (ALLO) is an activemetabolite of progesterone (P4) involved in physiological and reproductive parameters of female rat. Previously we showed thatALLO impact on the hypothalamic-pituitary- gonadal axis in response to stress and mood disturbances generatingmorphophysiological alterations in ovarian, being able to inhibit apoptosis and promote angiogenesis of corpora lutea. Furthermore,ALLO is able to inhibit ovulation and generate the formation of cystic structures. Epidemiological and in vitro studies show that P4has anti-tumor effects. However, the molecular mechanism of the anticancer effect of progestogen is not yet fully understood. And theeffect of ALLO in ovarian cancer is unknown. So we proposed that ALLO affect tumor progression. In this work, we analyzed theproliferation and migration of human ovarian tumor cells IGROV-1 and SKOV-3. For that ovarian cancer line IGROV-1 and SKOV-3 were exposed to different concentrations of ALLO (10 -11 -10 -7 M) for 72 h. We analyzed proliferation by MTS assay and migrationby wounded assay. ALLO increased proliferation in a concentration depend manner, reaching a maximum effect of 80% (p<0.001 vs.control: untreated cells). SKOV-3 had an inhibitory effect on proliferation of 20% to 10 -10 M ALLO (p <0.01 vs. control). ALLOincreased the migration of cancer cells IGROV-1 in 60 % to 10 -8 M (p<0.001 vs. control: untreated cells) and ALLO had the oppositeeffect in SKOV-3 line inhibiting in a 25% the migration to 10 -8 M (p <0.01 vs. control). We conclude that ALLO could modifyproliferation and migration of ovarian tumors affecting tumor progression. We consider it important to continue elucidating the effectof this steroid in the biology of ovarian cancer.