A Maternal Diet Enriched In Extra Virgin Olive Oil Does Not Prevent Reduced Uteri Weight and Decidual PPAR Levels in The Offspring of Diabetic Rats.

CINTIA ROMINA GATTI; SABRINA LORENA ROBERTI; DALMIRO GOMEZ RIBOT; ROMINA HIGA; ALICIA JAWERBAUM

Congreso; Latin American DOHaD 2020. One World One Health. On the Web; 2020

Background and Aim:Maternal diabetes increases the risks of maternal, fetal and placental complications and leads to the programming of metabolic and cardiovascular diseases in the offspring. These alterations are in part related to a maternal diabetes-induced prooxidant-proinflammatory intrauterine environment, which may alter the uterus in the female offspring. Our recent studies have shown that a dietary supplementation with extra virgin olive oil (EVOO) is able to reduce markers of the prooxidant-proinflammatory state in the placenta and different fetal organs in experimental models of diabetes and pregnancy. EVOO is highly enriched in oleic acid, a monounsaturated fatty acid that activates Peroxisome Proliferator Activated Receptors (PPARs). PPARs are involved in decidualization and anti-inflammatory processes. The aim of this work was to evaluate the uterus of the offspring of diabetic rats that receive or not an EVOO enriched diet during pregnancy, evaluating the weight of decidualized and not-decidualized uteri at a prepubertal age, as well as addressing PPARs expression in the decidua at a postimplantation stage. Methods:A mild pregestational diabetic rat model was induced in F0 females by neonatal administration of streptozotocin (90 mg/kg sc). Control and diabetic females were mated with healthy males and were fed or not with a 6% olive oil enriched diet from day 1 of pregnancy until parturition. The offspring were fed a standard diet and the uteri of the female offspring (F1) were evaluated a) at a prepuberal stage: 30 day postnatal, with or without induction of decidualization with PMSG (50 UI) and hCG (50 UI) and b) on day 9 of pregnancy. At the prepuberal stage non-decidualized and decidualized uteri were weighted, and on day 9 of pregnancy, the expression of PPARalpha, PPARgamma and PPARdelta was evaluated in the decidua. Results:At a prepubertal stage, the offspring of diabetic rats (Diabetic Group) showed a reduction in weight in the non-decidualized uteri (36%, p